An Expressed Sequence Tag collection from the male antennae of the Noctuid moth Spodoptera littoralis: a resource for olfactory and pheromone detection research.

摘要: Nocturnal insects such as moths are ideal models to study the molecular bases of olfaction that they use, among examples, for detection mating partners and host plants. Knowing how an odour generates a neuronal signal in insect antennae is crucial understanding physiological olfaction, also could lead identification original targets development olfactory-based control strategies against herbivorous moth pests. Here, we describe Expressed Sequence Tag (EST) project characterize antennal transcriptome noctuid pest model, Spodoptera littoralis, identify candidate genes involved odour/pheromone detection. By targeting cDNAs from male antennae, biased gene discovery towards potentially including pheromone reception. A total 20760 ESTs were obtained normalized library assembled 9033 unigenes. 6530 annotated based on BLAST analyses prediction software identified 6738 ORFs. The unigenes compared Bombyx mori proteome derived Lepidoptera projects. We large number turnover, 31 chemosensory receptor genes, but olfactory modulation. Our has generated collection transcripts Lepidoptera. normalization process, allowing enrichment low abundant proved be particularly relevant receptors species which no genomic data available. results suggest modulation can take place at level itself. These EST resources will invaluable exploring mechanisms S. ultimately identifying fight