VIDAS D-dimer: fast quantitative ELISA for measuring D-dimer in plasma.

摘要: VIDAS D-dimer (bioMerieux) is a new quantitative ELISA for determination designed the automated system. The test contains single-dose, ready-to-use reagents and completed within 35 min. Quantitative results are obtained from calibration curve stored in software of system expressed as fibrinogen equivalent units. two-step capture/tag relies on two complementary monoclonal anti-D-dimer antibodies, second one being labeled with alkaline phosphatase. upper limit measuring range 1000 micrograms/L lower detection <50 micrograms/L, which below reference interval (68-494 micrograms/L). Reproducibility (CV) between runs ranges 5% to 7%. There no interference heparin, bilirubin, hemoglobin, degradation products, or plasma turbidity. Comparison conventional (y) gave good correlation (r= 0.91, n= 579) comparable (y= 1.35x - 148, S(y/x)= 750), especially concentrations ranging 0 1.09x 10.6, r= 0.88, 170).