Human respiratory epithelial cell culture for drug delivery applications.

摘要: Abstract Recent developments in delivering drugs to the lung are driving need for vitro methods evaluate fate of inhaled medicines. Constraints on experimentation using animals have promoted use human respiratory epithelial cell cultures model absorption barrier lung; with two airway lines, 16HBE14o- and Calu-3, primary cultured alveolar type I-like cells (hAEpC) gaining prominence. These models develop permeability properties which comparable those reported native epithelia. This is contrast high A549 line, unsuitable drug experiments. Tabulation apparent coefficients ( P app ) compounds measured ‘absorptive’ ‘secretory’ directions reveals that fewer 50). Vectorial (asymmetric) transport three types P-glycoprotein, most studied mechanism, being all. Progress made towards vitro–in vivo-correlation pulmonary metabolism, toxicity targeting strategies. In summary, culture layers been established data regarding their characteristics suitability becoming available. Discerning circumstances under will be essential, or offers advantages over non-organ, non-species specific models, next challenge.