Mixed hematopoietic chimerism after allogeneic transplantation with lymphocyte-depleted bone marrow is not associated with a higher incidence of relapse.
作者： A Schattenberg , T De Witte , M Salden , J Vet , B Van Dijk
DOI: 10.1182/BLOOD.V73.5.1367.1367
关键词:
摘要: Using red cell phenotyping, cytogenetic analysis of blood lymphocytes, chromosome studies bone marrow cells, and restriction fragment length polymorphism (RFLP) peripheral we demonstrated a high number mixed chimeras after allogeneic transplantation (BMT). Donor from HLA-A, -B, -DR identical, lymphocyte culture (MLC) nonreactive siblings was depleted 98% lymphocytes using counterflow centrifugation. Thirty-two 48 recipients (67%) appeared to be at 6 months transplantation. The is probably result depletion the graft sensitivity phenotyping for demonstration minor populations (at levels as low 0.01%). probability relapse-free survival 4 years BMT 85% 65% complete donor chimeras. We conclude that in this study, chimerism not associated with higher incidence relapse.
ashpublications.org
bloodjournal.org参考文章(34)
MF Bertheas, D Maraninchi, M Lafage, J Fraisse, D Blaise, AM Stoppa, G Michel, CP Brizard, MH Gaspard, G Novakovitch, P Mannoni, P Viens, Y Carcassonne, Partial chimerism after T-cell-depleted allogeneic bone marrow transplantation in leukemic HLA-matched patients: a cytogenetic documentation. Blood. ,vol. 72, pp. 89- 93 ,(1988) , 10.1182/BLOOD.V72.1.89.89
J. M. Goldman, J. F. Apperley, R. E. Marcus, J. Hows, E. C. Gordon-Smith, C. D. Reid, D. Catovsky, D. A. G. Galton, Bone Marrow Transplantation for Patients in the Chronic Phase of Chronic Granulocytic Leukaemia: Apparent Low Incidence of Relapse within the First Two Years Post-BMT Springer Berlin Heidelberg. pp. 14- 15 ,(1985) , 10.1007/978-3-662-40457-7_7
ED Thomas, JW Singer, A Keating, R Ramberg, R McGuffin, JE Sanders, G Sale, PJ Fialkow, Long-term stable hematopoietic chimerism following marrow transplantation for acute lymphoblastic leukemia: a case report with in vitro marrow culture studies. Blood. ,vol. 62, pp. 869- 872 ,(1983) , 10.1182/BLOOD.V62.4.869.869
RS Hill, FB Petersen, R Storb, FR Appelbaum, K Doney, S Dahlberg, R Ramberg, ED Thomas, Mixed Hematologic Chimerism After Allogeneic Marrow Transplantation for Severe Aplastic Anemia Is Associated With a Higher Risk of Graft Rejection and a Lessened Incidence of Acute Graft-Versus-Host Disease Blood. ,vol. 67, pp. 811- 816 ,(1986) , 10.1182/BLOOD.V67.3.811.811
E B Hook, Exclusion of chromosomal mosaicism: tables of 90%, 95% and 99% confidence limits and comments on use American Journal of Human Genetics. ,vol. 29, pp. 94- 97 ,(1977)
RG Knowlton, VA Brown, JC Braman, D Barker, JW Schumm, C Murray, T Takvorian, J Ritz, H Donis-Keller, Use of Highly Polymorphic DNA Probes for Genotypic Analysis Following Bone Marrow Transplantation Blood. ,vol. 68, pp. 378- 385 ,(1986) , 10.1182/BLOOD.V68.2.378.378
Y. Nakamura, M. Culver, S. Gillilan, P. O'Connell, M. Leppert, G.M. Lathrop, J.-M. Lalouel, R. White, Isolation and mapping of a polymorphic DNA sequence pYNZ23 to chromosome 1 (D1S58) Nucleic Acids Research. ,vol. 15, pp. 9620- 9620 ,(1987) , 10.1093/NAR/15.22.9620
T. Caspersson, L. Zech, C. Johansson, E.J. Modest, Identification of human chromosomes by DNA-binding fluorescent agents Chromosoma. ,vol. 30, pp. 215- 227 ,(1970) , 10.1007/BF00282002
E. Donnall Thomas, C. Dean Buckner, Reginald A. Clift, Alexander Fefer, F. Leonard Johnson, Paul E. Neiman, George E. Sale, Jean E. Sanders, Jack W. Singer, Howard Shulman, Rainer Storb, Paul L. Weiden, Marrow Transplantation for Acute Nonlymphoblastic Leukemia in First Remission New England Journal of Medicine. ,vol. 301, pp. 597- 599 ,(1979) , 10.1056/NEJM197909133011109
Jane F. Apperley, Feyruz Rassool, Antonio Parreira, Colin G. Geary, Christine Harrison, Derek Stansfield, John M. Goldman, Philadelphia-positive metaphases in the marrow after bone marrow transplantation for chronic granulocytic leukemia. American Journal of Hematology. ,vol. 22, pp. 199- 204 ,(1986) , 10.1002/AJH.2830220211