Vibrio cholerae interactions with Mytilus galloprovincialis hemocytes mediated by serum components.

摘要: Edible bivalves (e.g., mussels, oysters) can accumulate large amount of bacteria in their tissues and act as passive carriers pathogens to humans. Bacterial persistence inside depends, at least part, on hemolymph anti-bacterial activity that is exerted by both serum soluble factors phagocytic cells (i.e., the hemocytes). It was previously shown Mytilus galloprovincialis contains opsonins mediate D-mannose-sensitive interactions between hemocytes V. cholerae O1 El Tor carry Mannose–Sensitive Hemagglutinin (MSHA). These enhance phagocytosis killing vibrios facilitating binding hemocytes. Since strains not carrying MSHA ligand (O1 classical, non O1/O139) are present coastal water be entrapped we studied whether mussel serum, addition directed towards MSHA, other components opsonization these bacteria. By comparing classical O1/O139 with ASW it found M. increase approximately two fold adhesion to, association Experiments conducted high low molecular mass fractions obtained ultrafiltration indicated compounds have higher than 5000 Da. Serum exposure temperature (80°C) abolished its opsonizing capability suggesting involved active protein nature. Further studies needed define chemical properties specificity bacterial ligands opsonins. This information will central only better understand ecology, but also improve current bivalve depuration practices properly protect human health.