Effect of HBO therapy on adipose-derived stem cells, fibroblasts and co-cultures: In vitro study of oxidative stress, angiogenic potential and production of pro-inflammatory growth factors in co-cultures1.

摘要: Background A key moderator of wound healing is oxygen. Wound a dynamic and carefully orchestrated process involving blood cells, cytokines, parenchymal cells (i.e. fibroblasts mesenchymal stem cells) extracellular matrix reorganization. Human adipose derived as well human produce soluble factors, exhibit diverse effects on inflammation anti response are involved in processes.Hyperbaric oxygen therapy an effective adjunct treatment for ischemic disorders such chronic infection or wounds. In vitro hyperbaric were presented many studies except those mono- co-cultures fibroblasts. Objective The aim this study was to investigate the Methods Mono- from established. These cultures exposed every 24 h five consecutive days. Measuring experiments performed first, third fifth day. Therapy expression VEGF, IL 6 reactive species investigated. Results After exposure oxygen, cell culturess showed significant increase VEGF after 3 5 All significantly reduced formation throughout experiments. IL-6 decreased during experiment mono-cultures co-cultures. contrast, skin overall increased IL-6. Conclusions Hyperbaric leads immunmodulatory proangiogenetic wound-like enviroment