In Situ Hybridization: Principles and Applications

摘要: In situ hybridization (ISH) has become an extremely useful tool for the clinical pathology laboratory to aid oncologists, geneticists, and infectious disease specialists in diagnosis treatment of their patients. ISH utilizes nucleic acid (DNA or RNA) probes assess intact cells various types genetic alterations. Common applications include its use detect cancer cytological specimens, chromosomal alterations resected tumor specimens that predict prognosis, response therapy certain microorganisms specimen types. is typically performed either with fluorescently labeled are subsequently visualized a chromogen such as diaminobenzidine. If probes, technique referred Fluorescence Situ Hybridization, FISH. Alternatively, if probe requires subsequent visualization chemical reaction produces colored at site (a chromogen), Chromogenic CISH.