A novel antigen of Mycobacterium tuberculosis and MPLA adjuvant co-entrapped into PLGA:DDA hybrid nanoparticles stimulates mucosal and systemic immunity.

摘要: Abstract Background Due to initiation of Mycobacterium tuberculosis infection via the mucosal tissue respiratory tract, intranasal administration new vaccines is highly regarded enhance immunity. Our outline was evaluation and systemic immune responses in BALB/c mice after nasal delivery HspX/EsxS fused antigen along with MPLA adjuvant entrapped PLGA:DDA hybrid nanoparticles. Methods In this study, double emulsion solvent evaporation method (w/o/w) used prepare different nanoparticle formulations containing protein MPLA. Three weeks last immunization mice, IgA antibody levels lavage IFN-γ, IL-4, IL-17 TGF-β cytokines supernatant cultured splenocytes also serum IgG1 IgG2a titers were evaluated using ELISA method. Results results indicated that vaccination nanoparticles loaded protein±MPLA, both without a prime dose BCG could provide efficient Th1, Th17, IgA, responses. Conclusion These findings demonstrate as carrier/adjuvant adjuvant, efficiently induce against antigen, alone or booster for BCG.