Determination and quantification of alpha,beta,gamma,delta-unsaturated aldehydes as pentafluorobenzyl-oxime derivates in diatom cultures and natural phytoplankton populations: application in marine field studies
作者： Thomas Wichard , Serge A. Poulet , Georg Pohnert
DOI: 10.1016/J.JCHROMB.2004.10.021
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摘要: Max Planck Institute for Chemical Ecology, Hans-Knoll-Str. 8, D-07745 Jena, Germany. Reactive alpha,beta,gamma,delta-unsaturated aldehydes and oxo-acids produced by marine diatoms upon cell damage interfere negatively with the reproduction success of their grazers. A simple, sensitive specific method based on gas-chromatography coupled to mass spectrometry (EI or CI/EC) was developed quantification these deleterious substances in laboratory diatom cultures natural phytoplankton populations. For aldehyde quantification, containing samples are damaged presence O-(2,3,4,5,6-pentafluorobenzyl) hydroxylamine hydrochloride (PFBHA.HCl) which leads an situ derivatisation without inhibition biosynthesis aldehydes. The oxime derivates were addition reacted N-tert-butyldimethylsilyl-N-methyl-trifluoracetamide (MTBSTFA).
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