Intron polymorphisms of MAGI-1 and ACSF2 and effects on their expression in different goose breeds

摘要: Abstract The goose is one of the most important waterfowl, having lowing laying rate. Previous studies have shown SNPs in introns MAGI-1 (Record-106975) and ACSF2 (Record-106582) significantly associated with egg production geese. However, mechanism those influencing remains unclear. In this study, three breeds (Yangzhou geese, Zhedong white Carlos geese) obviously different were selected, allele frequency distribution functions investigated. results suggested that was among (χc2 = 92.377, Pc = 2.29 × 10−22), C appearing at frequencies 0.29 Yangzhou geese 0.94 contrast, not breeds. Quantitative Reverse Transcription PCR (qRT-PCR) showed expression AG genotype individuals higher than AA GG genotype. For ACSF2, CC had both AC luciferase reporter analysis revealed site-directed mutation (A>C) drove activity. Further altered binding site transcription factor BARHL2. Binding BARHL2 to intron confirmed by electrophoretic mobility shift assay (EMSA) analysis. Thus, our findings A>C could promote regulating BARHL2, resulting differences performance, which provided molecular insights into effect polymorphism on performance