Molecular events associated with ciclosporin A-induced gingival overgrowth are attenuated by Smad7 overexpression in fibroblasts.

摘要: Sobral LM, Aseredo F, Agostini M, Bufalino A, Pereira MCC, Graner E, Coletta RD. Molecular events associated with ciclosporin A-induced gingival overgrowth are attenuated by Smad7 overexpression in fibroblasts. J Periodont Res 2012; 47: 149–158. © 2011 John Wiley & Sons A/S Background and Objective:  Ciclosporin A (CsA)-induced is attributed to an exaggerated accumulation of extracellular matrix, which mainly due increased expression transforming growth factor-β1 (TGF-β1). Herein, the vitro investigation effects Smad7, a TGF-β1 signaling inhibitor, CsA-induced matrix was performed. Material Methods:  The were assessed stable fibroblasts from normal gingiva. Smad7-overexpressing cells control incubated CsA, synthesis type I collagen, production activity MMP-2 cellular proliferation evaluated ELISA, zymography, curve, bromodeoxyuridine incorporation assay cell cycle analysis. CsA on viability apoptosis gingiva also evaluated. Western blot immunofluorescence for phospho-Smad2 performed measure activation signaling. Results:  Although treatment stimulated both fibroblasts, its markedly inhibited cells, as revealed low levels phospho-Smad2. In proliferation, collagen significantly blocked. blocked fibroblast via p27 regulation. Neither nor induced death. Conclusion:  data presented here confirm that related molecular suggest effective blocking these events, including activity.