Extracellular glycosaminoglycan changes in healthy and overgrown gingiva fibroblasts after cyclosporin A and cytokine treatments.

摘要: BACKGROUND: It has been demonstrated that cyclosporin A (CyA) blocks the immune system, acts on cytoskeleton and stimulates production of extracellular matrix (ECM) transforming growth factor-beta1 (TGF-beta1). This cytokine, such as factor-alpha (TGF-alpha), induces deposition glycosaminoglycans (GAG), proteoglycans collagen fibres in ECM. METHODS: In this work, we examined effect induced by CyA, TGF-beta1 TGF-alpha cultures healthy overgrown human gingival fibroblasts order to evaluate glycosaminoglycan, cytoskeletal changes behaviour after concanavalin (Con A) treatment. Moreover, biopsies Alcian blue histochemical staining electron transmission microscopy. RESULTS: Total sulphated GAG gingiva specimens derived fibroblast treated with CyA cytokines were significantly higher than controls. The action was increased (P < or = 0.01) compared a greater comparison TGF-beta1; microscopy showed ECM accumulation. agglutinations heterogeneity populations. CONCLUSIONS: Stimulation Con population had cell surface heterogeneity, could respond different way both cytokine stimulus. synthesis normal before treatment suggests possible genetic origin damage. As not all CyA-treated patients develop overgrowth, predisposition may explain responses