Strains of Klebsiella Pneumoniae from Intensive Care Unit Producing CTX-M-15 Extended Spectrum Beta Lactamases

摘要: Problem statement: Resistance to third generation cephalosporins due acquisition and expression of Extended Spectrum β-Lactamase (ESBL) among Gram-negative bacteria is on the increase. Infections involving extended spectrum beta lactamase are associated with significant morbidity mortality. Therefore, infections ESBL isolates continue pose a serious challenge infection management worldwide. Since screening for not common practice in hospitals Enugu state, this study was undertaken characterize genes K. pneumoniae strains from intensive care unit University Nigeria Teaching Hospital (UNTH) Enugu. Approach: Over period 29 months, 57 patients out 140 receiving treatment Hospitals were found be infected extended-spectrum β-lactamase-producing pneumoniae. Species identification performed by Standard Microbiology methods re-confirmed MALDI-TOF technology. Phenotypic characterization Beta Lactamase determined double disc synergy test presence specific PCR. Results: All producers positive PCR blaCTX-M-1 cluster sequencing, blaCTXM-15 present. Genotypic beta-lactamase producing showed that all carried CTX-M-15and SHV genes, 41(71.9%) aac (6’)-Ib-cr blaOXA-1, 19(33%) blaTEM. ISEcp1 upstream ORF 477 downstream blaCTX-M. strains. Random amplified polymorphic DNA analysis grouped into two clonal groups- A B majority belong group (n = 42). Conclusion: This shows first time ICU UNTH therefore strongly butresses need regular ESBL-producing clinical specimen other wards Nigerian hospitals.