Genes Encoding TEM-4, SHV-2, and CTX-M-10 Extended-Spectrum β-Lactamases Are Carried by Multiple Klebsiella pneumoniae Clones in a Single Hospital (Madrid, 1989 to 2000)

摘要: Over a 12-year period (1989 to 2000), 159 Klebsiella pneumoniae isolates harboring extended-spectrum beta-lactamases (ESBLs) (4.8% of the total number K. obtained) were recovered from 58 patients, who mainly hospitalized in intensive care and surgery units. For 62 representative 31 clonal types TEM-4 (n = 5), SHV-2 7), SHV2a 4), SHV-5 1), CTX-M-10 13), or CTX-M-9 1) identified by pulsed-field gel electrophoresis. This is first report document presence beta-lactamase pneumoniae. These previously Escherichia coli Spain. Only two five clones caused more than single case infection, with one them spreading for 9 months. A plasmid was detected among these clones. seven containing three four strains SHV-2a infection. Plasmids encoding unrelated. Four 13 found patient, 2 5 years apart. As isolates, we unable common transmissible genetic element that could explain polyclonal structure our isolates. Nevertheless, spread gene may be suggested conserved set noncoding polymorphisms sequences. Most ESBL-producing ephemeral, being poorly selected maintained hospital setting, but genes ESBL persisted successfully over recovered, probably as minority population metagenome.