Dynamic Regulation of AMPAR Phosphorylation In Vivo Following Acute Behavioral Stress.

摘要: The tuning of glutamatergic transmission is an essential mechanism for neuronal communication. α-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors (AMPARs) are ionotropic glutamate that mediate fast synaptic transmission. phosphorylation states specific serine residues on the GluA1 and GluA2 AMPAR subunits considered critical post-translational modifications regulate activity subcellular trafficking. While behavioral stress, via stress hormones, exerts alterations such processes, there have been conflicting data concerning influence in different brain regions, post-stress signaling mechanisms mediating these processes not well delineated. Here, we examined dynamics at three (ser831-GluA1, ser845-GluA1, ser880-GluA2) four regions [amygdala, medial prefrontal cortex (mPFC), dorsal hippocampus, ventral hippocampus] rat during hour following stress. We also tested impact corticosteroid receptor blockade phosphorylation. Both subunit exhibited elevated after yet administration antagonists curtailed effects only ser831-GluA1. In contrast, ser880-GluA2 displayed a time-dependent tendency early decreased (that was selectively augmented by mifepristone treatment amygdala mPFC stressed animals) followed increased later on. These findings show vivo regulation dynamic subunit-specific process, they provide support hypothesis ongoing role ser831-GluA1 interval.