Survivin mRNA in peripheral blood is frequently detected and significantly decreased following resection of gastrointestinal cancers.

摘要: Background and Objectives We evaluated if mRNA expression of survivin, an inhibitor apoptosis, can be used to detect circulating tumor cells in peripheral blood patients with various gastrointestinal cancers they decrease following complete surgical resection. Methods Blood samples from 40 cancer were analyzed prior resection by direct quantitative real-time reverse transcriptase-PCR (RT-PCR) assays. Results Survivin was pre-operatively detected 35 (88%). Post-operative survivin levels significantly lower than pre-operative 59% resected non-detectable 38% (Wilcoxon rank test: P < 0.04). Conclusions This is the first report showing that RT-PCR analysis technically feasible. Survivin fall might become a molecular marker for completeness resection. J. Surg. Oncol. 2007; 95:51–54. © 2006 Wiley-Liss, Inc.