Entamoeba histolytica: intracellular distribution of the sec61α subunit of the secretory pathway and down-regulation by antisense peptide nucleic acids

摘要: The Sec61α protein is defined as a highly conserved essential integral component of the endoplasmic reticulum in eukaryotic cells. We report detailed immunolocalization Entamoeba histolytica homologue subunit (EhSec61α), which shows an irregular pattern throughout cell and also found on surface, its effective down-regulation by means antisense peptide nucleic acids effects proliferation, subcellular distribution two virulence factors, ability trophozoites to cause liver abscess hamsters. Although levels are specifically decreased PNA-treated trophozoites, proliferate more slowly than controls, mobilization cysteine protease 5 amoebapore surface not significantly impeded capacity induce hamsters largely unaffected. implications these findings discussed context peculiar biology E. histolytica.