TRAP assists membrane protein topogenesis at the mammalian ER membrane
作者： Nicole Sommer , Tina Junne , Kai-Uwe Kalies , Martin Spiess , Enno Hartmann
DOI: 10.1016/J.BBAMCR.2013.08.018
关键词:
摘要: Membrane protein insertion and topogenesis generally occur at the Sec61 translocon in endoplasmic reticulum membrane. During this process, membrane spanning segments may adopt two distinct orientations with either their N- or C-terminus translocated into ER lumen. While different topogenic determinants proteins, such as flanking charges, polypeptide folding, hydrophobicity, have been identified, it is not well understood how and/or associated components decode them. Here we present evidence that translocon-associated (TRAP) complex involved vivo. Small interfering RNA (siRNA)-mediated silencing of TRAP HeLa cells enhanced topology effect mutating charges a signal-anchor, but increasing signal hydrophobicity. The results suggest role moderating 'positive-inside' rule.
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