Limiting Dilution Assays for the Separation, Characterization, and Quantitation of Biologically Active Particles and Their Clonal Progeny

摘要: Publisher Summary This chapter discusses the limiting dilution assays (LDA) for separation, characterization, and quantitation of biologically active particles their clonal progeny. LDAs detect binary responses generated by BAPs in individual vivo or vitro cultures within groups replicate that vary dose test preparation from which are sampled. Relative frequency LDAs, however, also incorporate an accompanying physicochemical assay a general type defined structural morphology other characteristics called physicochemically observable (POPs). In absolute number POPs is never known because any could conceivably be used to observe them not performed impracticality impossibility. As result unstable nature most biological preparations, it impractical repeatedly same sample. It possible population performing, either simultaneously serially, sequence on samples with one each