Bacteriorhodopsin in ice
作者： Joachim Heberle , Norbert A. Dencher
DOI: 10.1016/0014-5793(90)80864-F
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摘要: The photocycle and the proton pumping kinetics of bacteriorhodopsin, as well transfer rate protons from membrane surface into aqueous bulk phase were examined for purple membranes in water ice. In water, optical pH indicator pyranine residing monitors H+-release later than fluorescein covalently linked to extracellular BR. frozen state, however, responds ejected H+ fast attached BR, demonstrating that surface/bulk is ice no longer limiting. pumped appears at during transition intermediate L550 M412. Arrhenius plot M formation suggests translocated through protein via an ice-like structure.
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