In Situ Microbial Community Succession on Mild Steel in Estuarine and Marine Environments: Exploring the Role of Iron-Oxidizing Bacteria.

摘要: Microbiologically influenced corrosion (MIC) is a complex biogeochemical process involving interactions between microbes, metals, minerals, and their environment. We hypothesized that sediment-derived iron-oxidizing bacteria (FeOB) would colonize become numerically abundant on steel surfaces incubated in coastal marine environments. To test this, coupons were sediments over 40 days, samples taken at regular intervals to examine microbial community succession. The experiments conducted two locations: (1) brackish salt marsh stream (2) bay. analyzed DNA extracted from the MIC biofilms for bacterial diversity using high-throughput amplicon sequencing of SSU rRNA gene, site single cell sorted screened gene. quantified communities Zetaproteobacteria, sulfate-reducing (SRB), total archaea qPCR analyses. Zetaproteobacteria SRB identified data analyses collected throughout incubations also present adjacent sediments. At site, lower compared sediments, consistent with expected enrichment FeOB steel. Their numbers increased rapidly first 10 days. other known not detected sediments; however dramatically within ten days surface, although was nearly clonal. Iron oxyhydroxide stalk biosignatures observed earlier culture studies; this evidence qPCR, pyrosequencing, likely FeOB. In environment, members freshwater present, but absent fully site. This work indicates there successional pattern colonization being early colonizers; time matures include may help accelerate corrosion. shows reservoir sediment habitats, where they influence iron cycle, can or sources Fe(II) when available.