Measurements of antimicrobial drug concentrations in renal interstitial fluid using the diffusion chamber technique.

摘要: A technique is described to obtain renal interstitial fluid (RIF) from rabbits after implantation of diffusion chambers with permeable membranes 0.45 µ porosity in both kidneys. Pharmacokinetic studies were conducted two three weeks implantation. No difference gentamicin concentrations, as measured microbiologically, was seen between RIF withdrawn the left and right kidney at same points time. Simultaneous drug concentrations determined serum oral administration ampicillin or nalidixic acid intramuscular injection gentamicin. Ampicillin peaked hours about one fourth peak concentration hour. These curves crossed 3.45 hours. In RIF, maximum found again approximately level half an The 3.15 levels could be detected microbiologically RIF. collected urine, however, this for several sampling periods. Our results show that chamber can useful pharmacokinetic examination drugs, also respect their distribution