Plasma Protein Electrophoresis in Birds: Comparison of a Semiautomated Agarose Gel System With an Automated Capillary System

作者: Yannick Roman , Marie-Claude Bomsel-Demontoy , Julie Levrier , Daniel Chaste-Duvernoy , Michel Saint Jalme

DOI: 10.1647/2011-040

关键词:

摘要: Plasma agarose gel electrophoresis (AGE) is recognized as a very reliable diagnostic tool in avian medicine. Within the last 10 years, new techniques such capillary zone (CZE) have emerged human laboratory medicine but never been investigated birds. To investigate use of CZE birds and to compare it with AGE, plasma samples from 30 roosters (Gallus gallus), 20 black kites (Milvus migrans), racing pigeons (Columba livia) were analyzed by both AGE CZE. For 3 species studied, values determined well correlated for albumin beta gamma fractions whereas other differed significantly. Values alpha-3 fraction rooster, alpha-1 kite, alpha pigeon obtained prealbumin Repeatability reproducibility appeared higher than AGE. Although interpretation patterns seems produce results similar those some proteins present measured migrated found requires specific reference intervals much sample volume, this method has many advantages when compared including better repeatability analysis output.

参考文章(40)
Terry W Campbell, Christine Ellis, Avian and Exotic Animal Hematology and Cytology ,(1988)
Maja Zdelar-Tuk, Blanka Beer Ljubić, Zvonko Stojević, Suzana Milinković-Tur, Natalija Filipović, Changes in concentration and fractions of blood serum proteins of chickens during fattening Veterinarski Arhiv. ,vol. 77, pp. 319- 326 ,(2007)
Linda L. Werner, Drury R. Reavill, The Diagnostic Utility of Serum Protein Electrophoresis Veterinary Clinics of North America: Exotic Animal Practice. ,vol. 2, pp. 651- 662 ,(1999) , 10.1016/S1094-9194(17)30114-7
Jiro J. Kaneko, Charles E. Cornelius, Clinical biochemistry of domestic animals ,(1963)
Donald W. Zantop, Greg J. Harrison, Branson W. Ritchie, Linda R. Harrison, Avian Medicine: Principles and Application ,(1994)
Jacques Bienvenu, Maria Stella Graziani, François Arpin, Hélène Bernon, Cynthia Blessum, Cristina Marchetti, Gabriella Righetti, Manuela Somenzini, Gioacchino Verga, Francesco Aguzzi, Multicenter evaluation of the Paragon CZETM 2000 capillary zone electrophoresis system for serum protein electrophoresis and monoclonal component typing Clinical Chemistry. ,vol. 44, pp. 599- 605 ,(1998) , 10.1093/CLINCHEM/44.3.599
Xavier Bossuyt, Alex Mewis, Norbert Blanckaert, Interference of Radio-opaque Agents in Clinical Capillary Zone Electrophoresis Clinical Chemistry. ,vol. 45, pp. 129- 131 ,(1999) , 10.1093/CLINCHEM/45.1.129
Murray E. Fowler, Zoo and wild animal medicine ,(1978)
Maria del Pilar Lanzarot, Andrés Montesinos, Manuel Ignacio San Andrés, Casilda Rodríguez, María Victoria Barahona, HEMATOLOGICAL, PROTEIN ELECTROPHORESIS AND CHOLINESTERASE VALUES OF FREE-LIVING NESTLING PEREGRINE FALCONS IN SPAIN Journal of Wildlife Diseases. ,vol. 37, pp. 172- 178 ,(2001) , 10.7589/0090-3558-37.1.172
MGJ Feuilloley, A Merieau, N Orange, Applications bio-médicales de l'électrophorèse capillaire. M S-medecine Sciences. ,vol. 15, pp. 1419- 1426 ,(1999) , 10.4267/10608/1285