The molecular epidemiology of bovine rotaviruses circulating in Iran: a two-year study
作者： Farzaneh Pourasgari , Jérôme Kaplon , Shahla Karimi-Naghlani , Céline Fremy , Vahid Otarod
DOI: 10.1007/S00705-016-3051-0
关键词:
摘要: Bovine group A rotavirus (bovine RVA) is recognized as a major cause of severe gastroenteritis in newborn calves. The purpose this study was to estimate the prevalence and identify genotypes circulating bovine RVA diarrheic Two hundred fifty-three stool samples calves up 1 month old were collected from 42 industrial dairy farms two Iranian provinces during March 2010 February 2012. All screened for presence by RT-PCR, G P determined semi-nested multiplex RT-PCR assay. results indicated that 49.4 % (125 out 253) positive RVA. genotyping subset (n = 85) revealed G6 (55.3 %) G10 (43.5 P[5] (51.8 P[11] (27 most prevalent genotypes, respectively. G6P[5] dominant genotype (35.3 %), followed G10P[5], G10P[11] G6P[11], with rates 16.5 %, 15.3 10.6 Sequence analysis 20 VP7 four VP4 genes showed highest nucleotide sequence identity corresponding strains RVA/Cow-tc/GBR/UK/1973/G6P7[5] RVA/Cow-tc/USA/B223/XXXX/G10P[11]. reveal diversity expands our knowledge infections Middle East. These also highlight importance producing an effective vaccine its inclusion national cattle immunization program.
springer.com
archives-ouvertes.fr
sci-hub.se 参考文章(65)
Leonard A, Miller J, Gunson Rn, Carman Wf, Importance of PCR in the diagnosis and understanding of rotavirus illness in the community. Communicable disease and public health. ,vol. 6, pp. 63- 65 ,(2003)
YOSHIYUKI Suzuki, TAKESHI Sanekata, MITSUO Sato, KAZUHIKO Tajima, Y Matsuda, O Nakagomi, None, Relative frequencies of G (VP7) and P (VP4) serotypes determined by polymerase chain reaction assays among Japanese bovine rotaviruses isolated in cell culture. Journal of Clinical Microbiology. ,vol. 31, pp. 3046- 3049 ,(1993) , 10.1128/JCM.31.11.3046-3049.1993
Vera Gouvea, Norma Santos, M do C Timenetsky, None, Identification of bovine and porcine rotavirus G types by PCR. Journal of Clinical Microbiology. ,vol. 32, pp. 1338- 1340 ,(1994) , 10.1128/JCM.32.5.1338-1340.1994
V Gouvea, N Santos, M do C Timenetsky, VP4 typing of bovine and porcine group A rotaviruses by PCR. Journal of Clinical Microbiology. ,vol. 32, pp. 1333- 1337 ,(1994) , 10.1128/JCM.32.5.1333-1337.1994
A. Naghibi, G.R. Mohammadi, M. Rad, E.A. Afshari Safavi, A Case-Control Study of Association between Diarrhea in Newborn Calves and Infection with Rotavirus and Coronavirus in Some Industrial Dairy Herds of Mashhad Aarea, Iran in 2008 archives of razi institute. ,vol. 67, pp. 35- 41 ,(2012) , 10.22092/ARI.2016.103885
J R Gentsch, R I Glass, P Woods, V Gouvea, M Gorziglia, J Flores, B K Das, M K Bhan, Identification of group A rotavirus gene 4 types by polymerase chain reaction. Journal of Clinical Microbiology. ,vol. 30, pp. 1365- 1373 ,(1992) , 10.1128/JCM.30.6.1365-1373.1992
Mirjam Kühne Simmonds, George Armah, Richard Asmah, Indrani Banerjee, Susan Damanka, Mathew Esona, Jon R Gentsch, Jim J Gray, Carl Kirkwood, Nicola Page, Miren Iturriza-Gómara, None, New oligonucleotide primers for P-typing of rotavirus strains: Strategies for typing previously untypeable strains. Journal of Clinical Virology. ,vol. 42, pp. 368- 373 ,(2008) , 10.1016/J.JCV.2008.02.011
K Fukai, T Sakai, M Hirose, T Itou, Prevalence of calf diarrhea caused by bovine group A rotavirus carrying G serotype 8 specificity. Veterinary Microbiology. ,vol. 66, pp. 301- 311 ,(1999) , 10.1016/S0378-1135(99)00021-8
Omid Madadgar, Ahmad Nazaktabar, Hadi Keivanfar, Taghi Zahraei Salehi, Samad Lotfollah Zadeh, Genotyping and determining the distribution of prevalent G and P types of group A bovine rotaviruses between 2010 and 2012 in Iran. Veterinary Microbiology. ,vol. 179, pp. 190- 196 ,(2015) , 10.1016/J.VETMIC.2015.04.024
D.L. Swiatek, E.A. Palombo, A. Lee, M.J. Coventry, M.L. Britz, C.D. Kirkwood, Detection and analysis of bovine rotavirus strains circulating in Australian calves during 2004 and 2005. Veterinary Microbiology. ,vol. 140, pp. 56- 62 ,(2010) , 10.1016/J.VETMIC.2009.07.020