Responses of pertussis toxin-treated microvascular endothelial cells to transforming growth factor beta 1. No evidence for pertussis-sensitive G-protein involvement in TGF-beta signal transduction.

摘要: Responses of bovine adrenal capillary endothelial cells (BACE) on treatment with transforming growth factor beta 1 (TGF-beta 1) have been characterized and tested for sensitivity to inactivation pertussis toxin-sensitive G-proteins. TGF-beta elicited inhibition, monolayer remodeling, elevation steady state mRNA levels collagen type (alpha 1(1) alpha 2(1)) 1, inhibition p34cdc2 histone H1 kinase activity in BACE cells. Pertussis toxin enhanced both cell [3H]methylthymidine uptake remodeling monolayers by 1. These findings contrast studies mink lung epithelial cells, which has shown be pertussis-sensitive. Further investigation revealed that had no effect 1-stimulated or Analysis potent also abolished the increase activity, however, precluding determination this response Consistent suppression activation, caused substantial mitogen-stimulated uptake. It is concluded signal transduction does not involve G-proteins class that, view its effects DNA synthesis use determine G-protein involvement cytokine signalling pathways should approached caution.