Expression and hormone regulation of Wnt2, 3, 4, 5a, 7a, 7b and 10b in normal human endometrium and endometrial carcinoma.
作者： TD Bui , L Zhang , MCP Rees , R Bicknell , AL Harris
DOI: 10.1038/BJC.1997.195
关键词:
摘要: Wnt genes are transforming to mouse breast epithelium and hormonally regulated in vivo. To assess their role another endocrine-responsive human cancer, the expression of seven (Wnt 2, 3, 4, 5a, 7a, 7b 10b) normal endometrium endometrial cells, carcinoma tissues cell lines was investigated by ribonuclease protection analysis. Wnt2, 4 5a mRNAs but not Wnt7a, or 10b were expressed primary culture epithelial (NEE) stromal (NES) cells. In contrast, four (RL95-2, HEC-1-A, AN3 CA Ishikawa), Wnt2 Wnt3 absent. Wnt4 only one out (RL95-2), Wnt5a much lower. Wnt7a Wnt7b three HEC-1-A Ishikawa). Wnt10b mRNA RL95-2 CA. fresh tissues, all apart from carcinoma. Similar lines, level significantly higher than 3 also lower compared with endometrium. There no difference between proliferative phase secretory menstrual cycle, either first trimester pregnancy. vitro, progesterone and/or 17beta-oestradiol had effect on NES lines. The data indicate that six 7a 7b) endogenously endometrium, independent gene down-regulation as well may be associated
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