Hyperacute rejection in ex vivo-perfused porcine lungs transgenic for human complement regulatory proteins.

摘要: Summary Inhibition of complement activation via human membrane-associated regulators is known to prevent hyperacute rejection in heart and kidney pig-to-primate transplantation. The protective effect such strategies pulmonary xenografts, however, seems be insufficient. In an ex vivo perfusion, model lungs from donor pigs transgenic for CD55 (n = 6) or CD59 (n = 5) were perfused with fresh blood compared nontransgenic organs (n = 6). addition, a soluble component 1 esterase inhibitor (C1-Inh) was applied h-CD55 (n = 3). the group, survival prolonged (P < 0.05), quality maximal time oxygenation significantly improved vascular resistance reduced control group. There decreased sequestration platelets, less parenchymal injury deposition C5b−9 Additional inhibition did not prolong lungs. Survival function expressing h-CD59 different parameters observed this lung transplantation, membrane-based resulted function. However, minor histopathological injuries these xenografts suggested only partial protection dysfunction by alone.