T11TS inhibits glioma angiogenesis by modulation of MMPs, TIMPs, with related integrin αv and TGF-β1 expressions.
作者： Manoj Kumar Singh , Debanjan Bhattacharya , Suhnrita Chaudhuri , Sagar Acharya , Pankaj Kumar
DOI: 10.1007/S13277-013-1296-8
关键词:
摘要: During glioma development, angiogenesis plays a crucial role in growth and vascularization of primary brain tumors. T11 target structure (T11TS), bioactive molecule, has been documented as an anti-neoplastic agent glioma-induced rats also human vitro. This novel molecule induces apoptosis tumor cells by way immune potentiation impairs the cell cycle, but its not worked out detail. Matrix metalloproteinases (MMPs) are enzymes promoting enzymatically remodeling extracellular matrix altering surface protein expression such integrin αv matrix-bound proteins like TGF-β1. The present study was formulated to assess efficacy T11TS modulations MMP-2 −9 their endogenous inhibitors (TIMP-1 TIMP-2) well TGF-β1 on phenotypic markers endothelial (CD31 CD34). parameters used were zymography, western blot, flow cytometric analyses. It observed that administration significantly downregulates metalloproteinase-2 along with ligand upregulates TIMP-1 TIMP-2. In situ immunofluorescence FACS results revealed decreased (CD31/PECAM1, CD34), inhibiting grip downregulating (ELISA) from microglia microenvironment. These suggest suppresses positive angiogenic factors potentiates negative regulators glioma-associated (ECs), resulting anti-angiogenic effect angiogenesis.
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