An assessment of microbial coronal leakage in teeth root filled with gutta-percha and three different sealers.

摘要: Summary The aim of this in vitro study was to determine the effect three different root canal sealers on bacterial penetration obturated canals. Eighty extracted human teeth with straight, single canals were prepared using modified double-flared technique balanced force under copious irrigation until master apical file size 40. The divided randomly into experimental groups (60 teeth) and control (20 teeth). Twenty each by lateral condensation cold gutta-percha AH26, Apexit or Tubliseal EWT sealer. In groups, 10 same These completely sealed serve as negative controls. remaining not served positive surface tooth, except 2 mm, nail varnish. coronal part cut end a polypropylene tube placed glass bottle containing sterile Todd-Hewitt Broth (THB). Aliquots 0.5 ml THB injected model system centrifuged at 168 g. An innoculum Streptococcus sanguis added 5-day intervals daily observations made for growth reservoir 90 days. AH showed within 24 h remained uncontaminated throughout test period. Leakage through varied from 7 86 There no statistically significant difference (P>0.05) leakage between sealers.