Spatiotemporal-Controlled Reporter for Cell-Surface Proteolytic Enzyme Activity Visualization.
作者： Haolun Cheong , Jisu Kim , Jing Mu , Wenmin Zhang , Juan Li
关键词:
摘要: Live-cell imaging of cell-surface-associated proteolytic enzymes is crucial to understand their biological roles and functions in both physiological pathological processes. However, the complexity cell membrane environment increases difficulties specifically investigating targeted activities within microenvironment. Towards this end, a unique, photoremovable, furin-responsive peptide probe that can undergo spatiotemporal control through UV-light illumination has been designed synthesized aid visualizing activity protease enzyme, furin, live cells. Prior light irradiation, photolabile moiety, 4,5-dimethoxy-2-nitrobenzyl, sequence reporter will block furin-like enzymatic hydrolysis, thus, no fluorescence be observed. Upon simple illumination, photolysis occur, thereby revealing uncaged probe, which enzyme hydrolysis lead an increase signal; allows real-time endogenous function living cells precise spatial temporal resolution.
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