Valor nutritivo de la harina de lombriz (Eisenia foetida) como fuente de aminoácidos y su estimación cuantitativa mediante cromatografía en fase reversa (HPLC) y derivatización precolumna con o-ftalaldehído (OPA)

摘要: Earthworms (Eisenia foetida) are of nutritional interest since they a rich source protein (> 60% w/w). The aimof this work was to determine amino acid content in proteins from samples earthworm flour. acids wereseparated by reversed phase high performance liquid chromatography and detected through molecular fluorescencesubsequent derivatization with o-phthalaldehyde. accuracy precision the method developed were satisfactory,and linear response within concentration range 2 - 32 μM observed. flour characterizedby evaluating some essential acids, such as: phenylalanine, leucine, lysine, isoleucine, methionineand valine 3% findings agreement those earlier studies indicating thatthe analytical described paper may be adopted for analysis samples. Thisspecies could provide an answer ecological problems developing countries