Analysis of Alpha-2 Macroglobulin from the Long-Lived and Cancer-Resistant Naked Mole-Rat and Human Plasma

摘要: Background The naked mole-rat (NMR) is a long-lived and cancer resistant species. Identification of potential anti-cancer age related mechanisms great interest makes this species eminent to investigate strategies understand aging mechanisms. Since it known that the NMR expresses higher liver mRNA-levels alpha 2-macroglobulin than mice, nothing about its structure, functionality or expression level in compared human A2M. Results Here we show comprehensive analysis NMR- plasma-A2M, showing different prediction glycosylation NMR-A2M, which results molecular weight Additionally, found concentration A2M (8.3±0.44 mg/mL vs. 4.4±0.20 mg/mL) lower total plasma protein content (38.7±1.79 61.7±3.20 human. NMR-A2M can be transformed by methylamine trypsin resulting conformational change similar detectable polyclonal antibody against Determination tryptic anti-tryptic activity revealed plasma. On other hand, less proteolytic was Conclusion We binding specific receptor LRP1. could demonstrate LRP1 tissue but This accompanied EpCAM as central adhesion molecule progression. NMR-plasma capable increase fibroblast vitro most probably increasing CD29 expression. first report, demonstrating similarities well distinct differences between might directly linked intriguing phenotype suggests play an important role anti-aging NMR.