Mechanism of immunoprotective effects of curcumin in DLM-induced thymic apoptosis and altered immune function: an in silico and in vitro study
作者： Anoop Kumar , Dinakar Sasmal , Surender Singh Jadav , Neelima Sharma , None
DOI: 10.3109/08923973.2015.1091004
关键词:
摘要: Curcumin, a main component of Curcuma Longa Linn, is plant polyphenol used as an immune-enhancer in the Indian system traditional medicine. However, its underlying mechanism immune-protection remains unknown. The present study designed to delineate role curcumin deltamethrin (DLM)-induced thymocyte apoptosis and altered immune functions. In silico studies revealed that has strong binding affinity toward CD4 CD8 receptors. DLM (25 µM) induces thymocytes through oxidative stress caspase-dependent pathways. Various concentrations (1, 10 50 µg/ml), when added along with DLM, caused concentration- time-related amelioration apoptogenic signaling pathways induced by DLM. Inhibition DLM-induced reactive oxygen species production, replenishment glutathione suppression caspase activities may thus be responsible for downstream cascade events, i.e. apoptosis, phenotypic changes cytokine release. Thus, this clearly demonstrates immunoprotection thymic includes inhibition apoptosis.
sci-hub.se 参考文章(32)
T Fernandes-Alnemri, G Litwack, E S Alnemri, CPP32, a novel human apoptotic protein with homology to Caenorhabditis elegans cell death protein Ced-3 and mammalian interleukin-1 beta-converting enzyme. Journal of Biological Chemistry. ,vol. 269, pp. 30761- 30764 ,(1994) , 10.1016/S0021-9258(18)47344-9
Mehdi Shakibaei, Gundula Schulze-Tanzil, Thilo John, Ali Mobasheri, Curcumin protects human chondrocytes from IL-l1beta-induced inhibition of collagen type II and beta1-integrin expression and activation of caspase-3: an immunomorphological study. Annals of Anatomy-anatomischer Anzeiger. ,vol. 187, pp. 487- 497 ,(2005) , 10.1016/J.AANAT.2005.06.007
Wenbin Zhu, Meghan M. Cromie, Qingsong Cai, Tangfeng Lv, Kamaleshwar Singh, Weimin Gao, Curcumin and Vitamin E Protect against Adverse Effects of Benzo[a]pyrene in Lung Epithelial Cells PLoS ONE. ,vol. 9, pp. e92992- ,(2014) , 10.1371/JOURNAL.PONE.0092992
H. A. Abu-Rizq, M. H. Mansour, A. M. Safer, M. Afzal, Cyto-protective and immunomodulating effect of Curcuma longa in Wistar rats subjected to carbon tetrachloride-induced oxidative stress. Inflammopharmacology. ,vol. 16, pp. 87- 95 ,(2008) , 10.1007/S10787-007-1621-1
J.H. Kolaczinski, C.F. Curtis, Chronic illness as a result of low-level exposure to synthetic pyrethroid insecticides: a review of the debate. Food and Chemical Toxicology. ,vol. 42, pp. 697- 706 ,(2004) , 10.1016/J.FCT.2003.12.008
Jorge Regueiro, Maria Llompart, Carmen Garcia-Jares, Rafael Cela, None, Development of a high-throughput method for the determination of organochlorinated compounds, nitromusks and pyrethroid insecticides in indoor dust Journal of Chromatography A. ,vol. 1174, pp. 112- 124 ,(2007) , 10.1016/J.CHROMA.2007.08.052
Arpana Sood, Roy Mathew, Howard Trachtman, Cytoprotective Effect of Curcumin in Human Proximal Tubule Epithelial Cells Exposed to Shiga Toxin Biochemical and Biophysical Research Communications. ,vol. 283, pp. 36- 41 ,(2001) , 10.1006/BBRC.2001.4749
R.S. Ramsewak, D.L. DeWitt, M.G. Nair, Cytotoxicity, antioxidant and anti-inflammatory activities of curcumins I-III from Curcuma longa Phytomedicine. ,vol. 7, pp. 303- 308 ,(2000) , 10.1016/S0944-7113(00)80048-3
Carlo Riccardi, Ildo Nicoletti, Analysis of apoptosis by propidium iodide staining and flow cytometry Nature Protocols. ,vol. 1, pp. 1458- 1461 ,(2006) , 10.1038/NPROT.2006.238
Anoop Kumar, Neelima Sharma, None, Comparative efficacy of piperine and curcumin in deltamethrin induced splenic apoptosis and altered immune functions Pesticide Biochemistry and Physiology. ,vol. 119, pp. 16- 27 ,(2015) , 10.1016/J.PESTBP.2015.03.003