Assay of insulin-like activity by the isolated fat cell method. IV. The biological activity of proinsulin.

摘要: The effect of bovine proinsulin and related factors on the glucose metabolism isolated, rat, fat cells was studied. dose-response curve parallel to that insulin action two proteins showed an identical time course. activity single chain about 0.5 U/mg (as estimated from its ability increase conversion of14C-glucose to14CO2 or to14C-glycerides by cells) rose 17 after treatment with trypsin. — quantitatively recovered in presence proinsulin. Split 5 U/mg, which 18 Connecting peptide did not influence absence insulin. There no isolated cell incubation medium a similar molecule high biological activity. same rat epididymal pads as cells, there significant suppression Kunitz pancreatic trypsin inhibitor either system. following concluded: is 2 per cent insulin, caused itself. reason for low presumably smaller affinity receptors.