Thyrotropin-releasing hormone-diphtheria toxin-related polypeptide conjugates. Potential role of the hydrophobic domain in toxin entry.
作者： P Bacha , J R Murphy , S Reichlin
DOI: 10.1016/S0021-9258(18)33021-7
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摘要: Abstract We have separately coupled a modified thyrotropin-releasing hormone (TRH) molecule to two diphtheria toxin-related polypeptides, CRM26 and CRM45. Both polypeptides are enzymatically active but only CRM45 contains the hydrophobic domain of toxin molecule. The TRH-CRM45 conjugate caused 50% inhibition protein synthesis in GH3 rat pituitary cells at 3 X 10(-9) M. alone was 200 500 times less toxic than conjugate. Intoxication by prevented excess TRH, preincubation with antitoxin, or reduction disulfide cross-link. In addition, no more itself 3T3 which lack TRH receptors. contrast, TRH-CRM26, although it retained enzymatic activity, nontoxic for even 10(-7) Binding experiments showed that both conjugates compete receptors comparable affinities. potential role entry is discussed.
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sci-hub.st 参考文章(38)
D. Michael Gill, A.M. Pappenheimer, Structure-activity relationships in diphtheria toxin. Journal of Biological Chemistry. ,vol. 246, pp. 1492- 1495 ,(1971) , 10.1016/S0021-9258(19)76999-3
T Honjo, Y Nishizuka, O Hayaishi, I Kato, Diphtheria Toxin-dependent Adenosine Diphosphate Ribosylation of Aminoacyl Transferase II and Inhibition of Protein Synthesis Journal of Biological Chemistry. ,vol. 243, pp. 3553- 3555 ,(1968) , 10.1016/S0021-9258(18)93347-8
E C Heath, T N Oeltmann, A hybrid protein containing the toxic subunit of ricin and the cell-specific subunit of human chorionic gonadotropin. II. Biologic properties. Journal of Biological Chemistry. ,vol. 254, pp. 1028- 1032 ,(1979) , 10.1016/S0021-9258(17)34162-5
D W Chung, R J Collier, Enzymatically active peptide from the adenosine diphosphate-ribosylating toxin of Pseudomonas aeruginosa. Infection and Immunity. ,vol. 16, pp. 832- 841 ,(1977) , 10.1128/IAI.16.3.832-841.1977
Arthur M. Crestfield, Stanford Moore, William H. Stein, The preparation and enzymatic hydrolysis of reduced and S-carboxymethylated proteins. Journal of Biological Chemistry. ,vol. 238, pp. 622- 627 ,(1963) , 10.1016/S0021-9258(18)81308-4
P Boquet, A M Pappenheimer, Interaction of diphtheria toxin with mammalian cell membranes. Journal of Biological Chemistry. ,vol. 251, pp. 5770- 5778 ,(1976) , 10.1016/S0021-9258(17)33122-8
A. Schonbrunn, H. Tashjian, Characterization of functional receptors for somatostatin in rat pituitary cells in culture. Journal of Biological Chemistry. ,vol. 253, pp. 6473- 6483 ,(1978) , 10.1016/S0021-9258(19)46957-3
M Teng, J R Murphy, P Bacha, Determination of Corynebacterium diphtheriae toxigenicity by a colorimetric tissue culture assay. Journal of Clinical Microbiology. ,vol. 7, pp. 91- 96 ,(1978) , 10.1128/JCM.7.1.91-96.1978
Preparation of Concanavalin A-Ricin A-Chain Conjugate and Its Biologic Activity Against Various Cultured Cells Journal of the National Cancer Institute. ,vol. 62, pp. 1387- 1395 ,(1979) , 10.1093/JNCI/62.6.1387
F. Labrie, N. Barden, G. Poirier, A. De Lean, Binding of Thyrotropin-Releasing Hormone to Plasma Membranes of Bovine Anterior Pituitary Gland Proceedings of the National Academy of Sciences of the United States of America. ,vol. 69, pp. 283- 287 ,(1972) , 10.1073/PNAS.69.1.283