Histamine prevents radiation-induced mesenchymal changes in breast cancer cells.

摘要: Radiotherapy is a prime option for treatment of solid tumors including breast cancer though side effects are usually present. Experimental evidence shows an increase in invasiveness several neoplastic cell types through conventional tumor irradiation. The induction epithelial to mesenchymal transition proposed as underlying cause metastasis triggered by gamma Experiments were conducted investigate the role histamine on ionizing radiation-induced events cells with different invasive phenotype. We also evaluated potential involvement Src phosphorylation migratory capability irradiated upon treatment. MCF-7 and MDA-MB-231 mammary exposed single dose 2Gy radiation five days after irradiation mesenchymal-like phenotypic changes observed optical microscope. expression subcellular localization E-cadherin, β-catenin, vimentin Slug determined immunoblot indirect immunofluorescence. There was decrease marker E-cadherin β-catenin mainly localized cytoplasm. positive nuclei, matrix metalloproteinase-2 activity migration invasion significantly increased. In addition, significant enhancement phosphorylation/activation could be cells. received 1 or 20μM during 24h previous irradiated. Notably, pre-treatment prevented induced reduced behavior decreasing phospho-Src levels. Collectively, our results suggest that may block related open perspective use improve radiotherapy efficacy.