Glutamic acid racemase from Lactobacillusfermenti purification and properties

摘要: Abstract Since the discovery of alanine racemase (Wood and Gunsalus, 1951) , there have been reports a number amino acid racemases. Some them found to need pyridoxal phosphate as cofactor. Certain other racemases were not activated by this DPN has reported stimulate proline (Stadman Elliott, 1957) ATP AMP threonine (Amos, 1954) . Previously one authors that enzyme preparation Lactobacillus fermenti ATCC 9338 was able racemize several acids such glutamic acid, aspartic alanine, methionine, valine, isoleucine phenylalanine. Pyridoxal any stimulatory effect on rate racemization preparation, while some mentioned above apparently cofactor (Tanaka,1960) Evidence for occurence described investigators (Ayengar Roberts, 1952 ; Narrod Wood, Itoh, 1958 ). Glaser (1960) recently method purification from arabinosus However, few details properties given up present. The present communication is concerned with L. which proved be flavoprotein.