Identification of Thiolic Sarcolemmal Proteins as a Primary Target of Iron Toxicity in Cultured Heart Cells

摘要: Damage to the heart is one of most critical manifestations transfusional iron overload. Because at present no experimental model available simulate iron-induced disease in intact animal, we have employed rat myocardial cells culture for studying harmful effects and protective chelation(1-4). Our previous studies shown that toxicity myocyte cultures associated with profound, reproducible changes contractility electophysiologic behaviour (5,6); these abnormalities are a marked increase lipid peroxidation reflected change composition membrane lipids an increased production malondialdehyde (4,7); alterations may be prevented, or reversed by deferoxamine selective application pharmacologic stimulants such as caffeine calcium and; significantly modified use antioxidants α-tocopherol, ascorbic acid, simultaneous hypoxia.