Elimination of hepatitis C virus RNA in infected human hepatocytes by adenovirus-mediated expression of ribozymes.

摘要: Hepatitis C virus (HCV) infection is the cause of more than 90% chronic non-A non-B hepatitis worldwide (2). It has been estimated that 1% world population infected with HCV. Although transmission parenteral in majority cases, incidence and mechanisms nonparenteral remain uncertain. Viral variable; persistent viremia occurs about 60 to 70% 20 40% individuals ultimately develop cirrhosis, end-stage liver disease,and/orhepatocellularcarcinoma(2).Accordingtorecent data, HCV leading indication for transplantation United States, associated 50% hepatocellular carcinoma States (6a). Orthotopic performed patients disease, but persists 100% patients, ultimately, transplanted organ becomes reinfected (11). an enveloped positive-strand RNA a genome size approximately 9.4 kb (5, 13) which encodes single large open reading frame ;3,010 amino acids. The singlepolyproteingeneproductundergoesproteolyticprocessing multiple gene products, including capsid protein, two or envelope glycoproteins (E1 E2), six nonstructural products (NS2, NS3, NS4a, NS4b, NS5a, NS5b) are thought be involved viral assembly replication, respectively (10). After hepatocytes, genomic, positive strand translated. NS5 product, RNA-dependent polymerase, presumably catalyzes synthesis replicative, minus RNAstrandswhichrepresentthetemplateforproducingmore