Circular nucleic acid vectors, and methods for making and using the same

摘要: Circular nucleic acid vectors that provide for persistently high levels of protein expression are provided. The circular the subject invention characterized by being devoid expression-silencing bacterial sequences, where in many embodiments include a unidirectional site-specific recombination product hybrid sequence addition to an cassette. Also provided methods using introduction acid, e.g., cassette, into target cell, as well preparations use practicing such methods. and compositions find variety different applications, including both research therapeutic applications. is highly efficient readily scalable method producing employed methods, reagents kits/systems same.