Effect of catabolic hormone infusion on protein turnover and amino acid uptake in skeletal muscle.

摘要: Increased plasma levels of the catabolic hormones glucagon, epinephrine, and cortisol have been implicated in mediating various metabolic alterations trauma sepsis. Their role altered protein turnover amino acid transport skeletal muscle during sepsis, however, is not known. In current study, rats were infused with a mixture for 16 hours. Control animals vehicle solution. Protein synthesis degradation rates measured incubated, intact soleus muscles as incorporation 14 C-phenylalanine into release tyrosine incubation medium, respectively. Muscle uptake was determined by measuring intracellular to extracellular ratio [ 3 H]-α-aminoisobutyric after 2 Infusion hours resulted elevated glucose lactate levels, reduced concentrations most acids, accelerated breakdown, similar previous findings septic rats. incubated museles significantly different control hormone-infused The study suggests that increased proteolysis sepsis severe injury may be mediated part hormones. contrast, are probably signaled other substances or mechanisms.