A novel genome rearrangement involved in heterocyst differentiation of the cyanobacterium Anabaena sp. PCC 7120
作者： Andrey V. Matveyev , Eric Rutgers , Erik Söderbäck , Birgitta Bergman
DOI: 10.1111/J.1574-6968.1994.TB06701.X
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摘要: A new procedure for the preparation of intact microbial DNA allowed us to obtain DNA, suitable pulsed-field gel electrophoresis, from both vegetative cells and heterocysts (differentiated with a potential nitrogen fixation) cyanobacterium Anabaena PCC 7120. Through this it was possible locate genomic developmental rearrangements by visualizing increased mobility large heterocyst fragments undergoing rearrangements. The 390-kb SalI fragment cell shown lose about 70 kb as result previously reported 11- 55-kb deletions, restoring functional nif operons. rearrangement also detected. This takes place more than 600 upstream operons results in excision 18 505-kb fragment.
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