Imaging brain deoxyglucose uptake and metabolism by glucoCEST MRI.

摘要: 2-Deoxy-D-glucose (2DG) is a known surrogate molecule that useful for inferring glucose uptake and metabolism. Although (13)C-labeled 2DG can be detected by nuclear magnetic resonance (NMR), its low sensitivity detection prohibits imaging to performed. Using chemical exchange saturation transfer (CEST) as signal-amplification mechanism, the phosphorylated 2DG-6-phosphate (2DG6P) indirectly in (1)H (MRI). We showed CEST signal changed with concentration, was reduced suppressing cerebral metabolism increased general anesthetic. The changes were not affected or plasma pH, correlated altered blood flow demonstrated hypercapnia; neither they related extracellular amounts compared injection of D- L-glucose. In vivo (31)P NMR revealed similar 2DG6P suggesting reflected rate assimilation. This method provides new way use widely available MRI techniques image deoxyglucose/glucose without need isotopic labeling molecules.