Determination of amlodipine in pharmaceutical dosage forms by liquid chromatography and ultraviolet spectrophotometry

摘要: A liquid chromatography (LC) method and an ultraviolet (UV) spectrophotometric were developed validated for quantitative determination of amlodipine in tablets compounded capsules. The isocratic LC analyses performed on RP18 column using a mobile phase composed 0.1% (v/v) ortho-phosphoric acid (pH 3.0) -acetonitrile (60 + 40, v/v) at flow rate 1.0 mL/min. UV was 238 nm. analytical methods according to International Conference Harmonization Guidelines. calibration graphs linear [correlation coefficient (r) > 0.999] the studied concentration range 10-30 microg/mL 10-35 spectrophotometry. relative standard deviation values intraday interday precision studies less than 2%, accuracy greater 98% both methods. specificity proved forced degradation. Statistical showed no significant difference between results obtained by 2 proposed are precise accurate can be applied directly easily oral pharmaceutical preparations amlodipine.