Studies on the Organogenesis in Carrot Tissue Cultures II. Effects of Amino Acids and Inorganic Nitrogenous Compounds on Somatic Embryogenesis

摘要: Summary The effects of nitrogenous compounds on organogenesis in Daucus carota L. cv. US-Harumakigosun cultured vitro were examined. Reduced like ammonium nitrate or chloride with added such as potassium very effective inducing somatic embryogenesis, but sulfate was not. A high level a medium (without reduced nitrogen) slightly stimulated it. process embryogenesis seems to consist two sequential phases: the induction phase and development phase. former requires 2,4-D, not any compounds, while latter nitrogen. different L-amino acids embryo formation suspension cultures also When various amino individually basal which contained (20 mM) principal source nitrogen, α-alanine most at higher concentration (5 10 mM), glutamine, asparagine, aspartic acid, glutamic arginine proline stimulatory, lysine, valine, histidine, leucine methionine effective. β-alanine did stimulate pyruvic acid counteracted stimulative effect acid. addition accelerated cell division during earlier stage increased number embryos per cluster. Of without glutamine most; a-alanine concentrations (10 30 mM). Arginine, threonine seemed be stimulation absence other compound.