Salivary pellicles on titanium and their effect on metabolic activity in Streptococcus oralis.

摘要: Titanium implants in the oral cavity are covered with a saliva-derived pellicle to which early colonizing microorganisms such as Streptococcus oralis can bind. The protein profiles of salivary pellicles on titanium have not been well characterized and proteins importance for binding thus unknown. Biofilm bacteria exhibit different phenotypes from their planktonic counterparts contact may be one factor contributing induction changes physiology. We surfaces investigated how uncoated saliva-coated affects metabolic activity adherent cells S. oralis. Salivary smooth were desorbed these, purified human saliva, subjected two-dimensional gel electrophoresis mass spectroscopy. A parallel plate flow-cell model was used study fresh isolate surfaces. Metabolic assessed using Bac Light CTC Vitality Kit confocal scanning laser microscopy. Experiments carried out triplicate results analyzed Student’s t-test or ANOVA. Secretory IgA, α-amylase cystatins identified dominant pellicles. Selective adsorption demonstrated by enrichment prolactin-inducible absence zinc-α2-glycoprotein relative saliva. Adherence led an up-regulation population after 2 hours. In presence pellicle, this effect enhanced sustained over following 22 hour period. shown that adherence under flow causes colonizer oralis, most likely part adaptation biofilm mode life. containing sIgA, α-amylase, was, first time, abundant component titanium. Further studies needed clarify mechanisms underlying surface identify responsible enhancing effect.