Implication of 5-HT2A subtype receptors in DOI activity in the four-plates test-retest paradigm in mice.

摘要: Abstract The four-plates test (FPT) is an animal model of anxiety which allows the detection anxiolytic effect not only benzodiazepines (BZDs) but also other non-BZDs compounds such as antidepressants (ADs). Furthermore, DOI, a 5-HT2A/2C agonist, has been shown to exert anxiolytic-like in this model. Retesting mice models (test–retest paradigm) induces anxiogenic-like and loss effects response BZDs ADs. On contrary, DOI reported oppose fear potentiation induced by trial 1 FPT. Despite considered one most selective 5-HT2A available, it acts agonist at all three 5-HT2 receptor subtypes (5-HT2A, 5-HT2B 5-HT2C). aim study was thus investigate FPT test–retest paradigm, subtype(s) involved DOI-induced experienced mice. (0.25–4 mg/kg) agonists, 5-HT2B, BW 723C86 (1–16 mg/kg) 5-HT2C, RO 60-0175 have studied. Then, antagonism studies were conducted combinating antagonist SR 46349B, 5-HT2B/2C SB 206553 or 5-HT2C RS 10-2221 (at doses 0.1 1 mg/kg) with (1 mg/kg). Our shows that had no on retesting mice, whereas exerted naive By contrast neither nor 46349B antagonized anti-punishment effect. Diazepam included positive control increased each case number punished passages findings altogether suggest exerts its paradigm through receptors.