Identification of lily cultivars using isoelectric focusing of proteins from bulb scales and tissue culture bulblets

摘要: An efficient procedure is described for cultivar identification in lily, based on polymorphism bulb scale proteins analysed by isoelectric focusing (IEF). Seventy (84%) out of 83 cultivars from which vivo bulbs were showed a unique banding pattern. Analysis vitro bulblets 62 commercial resulted 47 (76%) patterns. Cultivars with identical protein patterns always belonged to the same classification group (i.e.. Asiatic hybrids, Oriental hybrids or Lilium longiflorum). Protein bands characteristic groups have been found. The appeared be very consistent and not affected soil-type grown, long-term storage, different sample storage methods, growing conditions, bulblet storage. However, six 10 analysed, consisted greater number of, and/or more intensely stained than those cultivar. unsuitable markers selection reference distinctness, uniformity stability (DUS) testing as no relation was found between morphological characters similar generally clearly distinct basis characters.