Isotopic Labeling of Proteins for Electrophoretic Analysis
作者： Bonnie S. Dunbar
DOI: 10.1007/978-1-4613-1957-3_6
关键词:
摘要: One of the most commonly used methods for high-resolution two-dimensional polyacrylamide gel electrophoresis (PAGE) has been analysis proteins during their synthesis in vivo or vitro cell tissue culture translation systems. These can also be to analyze undergoing posttranslational modification, e.g., glycosylation. The high specific activity [35S]methionine made this a popular reagent radiolabeling and is sensitive method many variety experimental Other radiolabeled amino acids mixes several these have successfully proteins. As with other protein identification, isotopic labeling certain advantages limitations. It important appreciate factors all designs.
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