Up-dated catalogue of HeLa cell proteins: percentages and characteristics of the major cell polypeptides labeled with a mixture of 16 14C-labeled amino acids.

摘要: A total of 1357 polypeptides [946 acidic (isoelectric focusing) and 411 basic (nonequilibrium pH-gradient electrophoresis)] from human HeLa cells have been separated catalogued with use high-resolution two-dimensional gel electrophoresis. Of these polypeptides, 1266 were detected by labeling [35S]methionine, while the rest revealed silver staining or a mixture 16 14C-labeled amino acids. For convenience, all numbered are indicated in large fold-out protein map. The percentages some major proteins determined, for we list few characteristics such as: variation during cell cycle; cellular distribution cytoplasts karyoplasts; presence Triton- salt-extracted cytoskeletons; phosphorylation sensitivity to neoplastic transformation.